Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TIPRL in samples. An antibody specific for TIPRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TIPRL is an inhibitory regulator of protein phosphatase-2A (PP2A), PP4 , and PP6. TIP isoform-2 did not interact with PP2A, suggesting that the C terminus of TIP is important for PP2A binding. Incubation of PP2A with TIP, but not TIP isoform-2, resulted in dose-dependent inhibition of PP2A phosphatase activity. Cells in which TIP was depleted by small interfering RNA exhibited decreased phosphorylation of a 32-kD substrate of ATM/ATR kinases.
TIP is an inhibitory regulator of PP2A and that the TIP/PP2A complex has a role within the ATM/ATR signaling pathway controlling DNA replication and repair.The predicted protein contains 272 amino acids. Immunoblot analysis showed ubiquitous expression of a 33-kD TIP protein.
