Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPOCK3 in samples. An antibody specific for SPOCK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The cDNA was a splice variant of SPOCK3, which the authors called testican-3, and included the N-terminal 313-amino acid region of SPOCK3 with a 3-amino acid substitution at the C terminus; the authors designated the splice variant N-TES. N-TES contains a signal peptide, a unique domain, a follistatin-like domain, and a Ca(2+)-binding domain, but lacks a C-terminal thyroglobulin domain and 2 putative glycosaminoglycan attachment sites of SPOCK3. The full-length SPOCK3 protein contains 436 amino acids and shares 51% and 44% homology with SPOCK1 and SPOCK2 , respectively. Semiquantitative RT-PCR detected expression of SPOCK3 and N-TES transcripts in normal brain; transcripts of both were downregulated in glioma tissues.
