Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPON1 in samples. An antibody specific for SPON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Spondin-1 is a protein contains 807 aminoacids and is structurally composed of six thrombospondin domains, one reelin domain, and one spondin domain.The deduced 624-amino acid partial SPON1 protein shares 96.8% amino acid sequence identity with the rat F-spondin precursor across 624 residues.SPON1 expression in lung, lower expression in brain, heart, kidney, liver, and testis, and lowest expression in pancreas, skeletal muscle, and ovary; no expression was found in spleen. In vitro binding assays using mutated human proteins confirmed that SPON1 specifically bound to the central APP domain (CAPPD). SPON1 inhibited APP cleavage by BACE1, the primary beta-secretase involved in APP processing. Binding also impaired APP- and FE65-dependent transactivation of the chromosome remodeling factor TIP60 .
