Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPP2 in samples. An antibody specific for SPP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP2 catalyzes the degradation of S1P.The deduced 399-amino acid protein has a calculated molecular mass of 44.7 kD and shares 39.3% amino acid identity with SGPP1. SGPP2 has an N-terminal hydrophobic region, 3 conserved phosphatase-family motifs, and is predicted to be an integral membrane protein with as many as 9 membrane-spanning segments. Immunofluorescence microscopy showed a reticular staining pattern in the perinuclear and cytosolic regions of HEK293 cells, and SGPP2 colocalized with an endoplasmic reticulum antibody. Northern blot analysis of human tissues detected a 5.1-kb transcript with high expression in kidney and heart, followed by brain, colon, lung, and small intestine.
