Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPRN in samples. An antibody specific for SPRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SPRN expression in human, rat, and mouse brain. The deduced human protein contains 151 amino acids. The mammalian proteins share 81 to 95% sequence identity. Alignment of all fish and mammalian Sho proteins showed that all have an N-terminal peptide sequence with an endoplasmic reticulum targeting signal for extracellular transport, a basic RG-rich region, a hydrophobic stretch in the middle of the protein that contains the same unusual composition of small aliphatic residues (GAV) as PrP and PrP-like proteins, and a C-terminal region with a putative N-glycosylation site and a possible GPI anchor site (Premzl et al., 2003, 2004). Database searches revealed that SPRN is expressed in mouse and rat embryo, brain, and retina, in human hippocampus, and in zebrafish embryo and retina.
