Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPTAN1 in samples. An antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sj?gren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.
