Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SPTBN4 in samples. An antibody specific for SPTBN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTBN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTBN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTBN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits.
SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.
