Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SSH3 in samples. An antibody specific for SSH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L, SSH1S, and SSH1B, and SSH2 encodes 2 isoforms, SSH2 and SSH2B. The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs (domains A and B) and are unique to the SSH family.
