Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate STAT4 in samples. An antibody specific for STAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. For further information on STATs. STAT4 and STAT1, which both map to chromosome 2q32, may have arisen via a tandem gene duplication. However, STAT1 was expressed ubiquitously, whereas STAT4 was expressed in specific tissues, including spleen, heart, brain, peripheral blood cells, and testis. STAT4 expression was drastically increased in T cells following treatment with a DNA methyltransferase inhibitor. Truncation of methylation sites in the proximal regulatory elements of the STAT4 promoter markedly enhanced transcriptional activity. The N-terminal protein interaction domain (N domain) of STAT4 is required for STAT4 activation after IL12 signaling.
