Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate STAT5B in samples. An antibody specific for STAT5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Signal transducer and activator of transcription 5B is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL).
