Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate STK39 in samples. An antibody specific for STK39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTK39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STK39 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STK39 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The deduced 547-amino acid protein has a calculated molecular mass of 59.6 kD. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detected ubiquitous expression of a 3.8-kb transcript, with most abundant expression in brain and pancreas. Western blot analysis of mouse tissues detected a protein of about 60 kD in all tissues examined.
