Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SULT1A1 in samples. An antibody specific for SULT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. PST is widely distributed in human and animal tissues that include the blood platelets. Human platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984). Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.
