Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SYCP3 in samples. An antibody specific for SYCP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYCP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYCP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYCP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SYCP3 encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein.
Three meiosis-specific components of the SC have been characterized in mammals, SC protein-1 (SYCP1), SYCP2, and SYCP3. SYCP3 has a molecular mass of 30 kD and a C-terminal coiled-coil domain that has been shown to promote homotypic interactions in vitro; the SYCP3 gene has been cloned in mouse, rat, and hamster.
