Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC9A3 in samples. An antibody specific for SLC9A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC9A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC9A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC9A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:An apical membrane Na+/H+ exchanger is involved in transepithelial, electroneutral Na+ absorption across renal and intestinal epithelia. The apical Na+/H+ exchange activity is pharmacologically distinct from the basolateral Na+/H+ exchange activity, notably in terms of response to kinase regulation and in addition by the diuretic amiloride. NHE1 (SLC9A1), the first of these exchangers to be cloned, localizes by immunocytochemical studies only to the basolateral membranes of polarized epithelial cells. Transfection of OKP cells with dominant-negative PYK2 or small interfering PYK2 duplex RNA blocked acid activation of NHE3, whereas neither had an effect on glucocorticoid activation of NHE3. Dominant-negative PYK2 also blocked acid activation of SRC kinase , which is required for acid regulation of NHE3.
