Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC7A2 in samples. An antibody specific for SLC7A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The gene encodes a 658-amino acid polypeptide that is 60% similar to CAT1 (ATRC1). CAT1 and CAT2 are members of the APC family of transporters, the members of which are specific for amino acids, polyamines, and choline. Northern blot analysis revealed that CAT2 is expressed as a 9.0-kb transcript in a variety of tissues.
CAT2A and CAT2B are produced by alternative splicing. The deduced CAT2A and CAT2B proteins contain 657 and 658 amino acids, respectively, and both have 14 transmembrane domains and 2 putative N-glycosylation sites in extracellular loop 3. They differ from one another in 19 residues in the vicinity of transmembrane segments 8 and 9.
