Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC7A10 in samples. An antibody specific for SLC7A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SLC7A10, in association with 4F2HC (SLC3A2), mediates high-affinity transport of D-serine and several other neutral amino acids.The deduced 530-amino acid protein contains 12 transmembrane regions and a conserved cysteine residue (cys160) in the putative extracellular loop between transmembrane domains 3 and 4. This residue was presumed to be linked to 4f2hc via a disulfide bond. Northern blot analysis detected a 1.9-kb transcript in mouse brain and lung, with weaker expression in placenta. A single 4.4-kb transcript was expressed in small intestine.ASC1 shares 91% identity with mouse Asc1. Northern blot analysis detected 1.3-, 2.0-, 4.3-, and 6.0-kb transcripts in brain, heart, placenta, skeletal muscle, and kidney. The 2.0-kb transcript predominated in brain.
