Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC7A1 in samples. An antibody specific for SLC7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Susceptibility to murine ecotropic retroviruses is attributed to the binding of the virus envelope to the membrane receptor encoded by the Rec1 gene.?HCAT1?was identified as the principal transporter of the cationic amino acids, arginine, lysine, and ornithine in mouse cells. the Rec1 gene mapped to mouse chromosome 5 by analysis of murine-hamster somatic cell hybrids. Using a human cDNA obtained by homology to Rec1, Albritton et al. (1992) determined the location of the human cationic amino acid transporter by somatic cell genetics, in situ hybridization, and RFLP linkage analysis. The studies indicated that ATRC1 is located at 13q12-q14, closely linked to ATP1AL1. The CEPH consortium linkage map of chromosome 13 published by Bowcock et al. (1993) showed ATRC1 to be distal to ATP1AL1.
