Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC6A4 in samples. An antibody specific for SLC6A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The serotonin transporter (SERT) is a monoamine transporter protein.This protein is an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamine, cocaine, and MDMA, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
