Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC6A19 in samples. An antibody specific for SLC6A19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SLC6A19 is a system B(0) transporter that mediates epithelial resorption of neutral amino acids across the apical membrane in the kidney and intestine.The deduced 634-amino acid protein contains 12 transmembrane domains. In situ hybridization detected Slc6a19 expression in renal cortical proximal tubules and in small intestine villus enterocytes, with highest expression in apical cells. EST database analysis suggested expression in several other tissues and cells. contains 634 amino acids and has 12 predicted transmembrane regions. In a panel of human cDNAs, they detected robust expression of SLC6A19 in kidney and small intestine, with minimal expression in pancreas. SLC6A19 was also expressed in stomach, liver, duodenum, and ileocecum.
