Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC5A7 in samples. An antibody specific for SLC5A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Choline is a direct precursor of acetylcholine (ACh), a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons.The deduced 580-amino acid protein has a calculated molecular mass of 63.2 kD.
CHT has 13 transmembrane (TM) domains, a short extracellular N terminus, and a long cytoplasmic C terminus. It contains 3 putative N-glycosylation sites and several phosphorylation sites. CHT shares 93% amino acid identity with rat Cht and 51% amino acid identity with its nematode homolog, Cho1.
