Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC5A1 in samples. An antibody specific for SLC5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters'. The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.The SLC5A1 protein contains a core of 13 transmembrane domains, which it shares with other members of the gene family, and it contains an additional transmembrane appended to the C termin.
