Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC39A4 in samples. An antibody specific for SLC39A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC39A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC39A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC39A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SLC39A4 gene as a candidate intestinal zinc-specific transporter within the susceptibility locus for the zinc-deficiency type of acrodermatitis enteropathica (AEZ) on 8q24.3. The gene is expressed as an mRNA of 2.5 kb in only a few tissues, i.e., kidney, colon, duodenum and jejunum. The duodenum and jejunum are crucial sites of zinc absorption. These transcripts encode proteins of 647 and 622 amino acids sharing the same C-terminal 583 residues. Computational analysis predicted that the larger protein has a cytoplasmic membrane location and a secondary structure characteristic of a ZIP protein, consisting of 8 transmembrane domains organized in 2 blocks of 3 and 5, respectively. These 2 blocks are separated by a variable intracytoplasmic metal-binding site.
