Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC34A1 in samples. An antibody specific for SLC34A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC34A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC34A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC34A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Magagnin et al. (1993) cloned from human kidney cortex a cDNA encoding a renal proximal tubular, brush-border membrane Na(+)-phosphate cotransporter referred to as NaPi3. Hartmann et al. (1996) reported the cloning and characterization of the mouse (Npt2) and human (NPT2) genes. The genes were cloned by screening mouse genomic and human chromosome 5-specific libraries, respectively.Hartmann et al. (1996) determined that the mouse and human NPT2 genes are approximately 16 kb and contain 13 exons Putative CAAT and TATA boxes were located, respectively, at positions -147 and -40 of the Npt2 gene and -143 and -51 of the NPT2 gene, relative to nucleotide 1 of the corresponding cDNAs. The translation initiation site was within exon 2 of both genes.
