Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC30A7 in samples. An antibody specific for SLC30A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc.The deduced proteins share 96% amino acid identity. Mouse Znt7 contains 6 transmembrane domains, a histidine-rich loop between transmembrane domains 4 and 5, cytoplasmic N and C termini, several phosphorylation sites, and an N-glycosylation site. Northern blot analysis of mouse tissues detected highest expression in liver, spleen, and small intestine. In rat epithelial and human fibroblast cell lines, ZNT7 clustered at the perinuclear region and showed punctate staining in the cytoplasm, indicative of cytoplasmic vesicle localization.
