Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC30A4 in samples. An antibody specific for SLC30A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Zinc is the second most abundant trace metal in the human body. It is an essential element, serving both a structural role, as in the formation of zinc fingers in DNA-binding proteins, and a catalytic role in metalloenzymes, such as pancreatic carboxypeptidases, alkaline phosphatases, various dehydrogenases, and superoxide dismutases. SLC30A4, or ZNT4, belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures, consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop . The gene encodes a 430-amino acid protein that is homologous to Znt2 (SLC30A2) and Znt3 (SLC30A3). Znt4 was abundantly expressed in mammary epithelia and brain.
