Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC27A5 in samples. An antibody specific for SLC27A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC27A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC27A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC27A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Very long chain fatty acid synthetase (VLACS, or SLC27A2), an enzyme whose function is presumed missing in ALD, is highly expressed in liver and kidney, but is expressed at much lower levels in brain. However, brain contains high amounts of VLCFA and is the organ most affected in ALD. In an attempt to isolate a VLACS isoform with a different tissue distribution, Berger et al. (1998) used degenerate PCR to clone a mouse cDNA and a partial human cDNA encoding a VLACS-related protein, termed VLACSR. VLACSR is 43% identical to mouse VLACS and 37% identical to mouse FATP. Northern blot analysis of mouse tissues revealed that a 2.6-kb VLACSR mRNA was highly abundant in liver. Smaller VLACSR transcripts were present at low levels in brain, lung, testis, spleen, and skeletal muscle.
