Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC25A20 in samples. An antibody specific for SLC25A20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC25A20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Carnitine-acylcarnitine translocase is one of several closely related mitochondrial-membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. This protein mediates the transport of acylcarnitines into mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway.
Mutations in this gene are associated with carnitine-acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
