Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC1A1 in samples. An antibody specific for SLC1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SLC1A1 encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
