Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC12A7 in samples. An antibody specific for SLC12A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC12A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC12A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC12A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By searching EST databases, Mount et al. (1999) identified a cDNA encoding SLC12A7, which they initially termed KCC3 but later renamed KCC4. The deduced 1,083-amino acid SLC12A7 protein contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C terminus, and 4 potential N-glycosylation sites.
SLC12A7 shares 65% amino acid identity with SLC12A4 and 66% identity with SLC12A6 . Northern blot analysis detected a 5.3-kb SLC12A7 transcript in most tissues tested, with highest expression in heart and kidney and little or no expression in adult brain. Functional analysis confirmed that SLC12A7 is a KCC.
