Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AKT3 in samples. An antibody specific for AKT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.Mice lacking Akt3 have a normal glucose metabolism (no diabetes), have approximately normal body weight, but have a 25% reduction in brain mass. Incidentally, Akt3 is highly expressed in the brain.
