Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SKIL in samples. An antibody specific for SKIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKIL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKIL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling.
