Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SIRPB2 in samples. An antibody specific for SIRPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described.
The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested.
