Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SFTPA1 in samples. An antibody specific for SFTPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SP-A (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of SP-A, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. SP-A (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of SP-A is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition. Genetic variations in SFTPA1 are associated with respiratory distress syndrome in premature infants (RDS) ; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants.
