Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SESN1 in samples. An antibody specific for SESN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySESN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SESN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SESN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Only the smaller transcript was transiently induced by p53 or by genotoxic agents in a p53-dependent manner in a colon carcinoma cell line. The deduced proteins, which are 99% identical to the mouse proteins, contain 551, 491, and 426 amino acids, respectively. RT-PCR analysis detected induction of T2 in response to p53 or genotoxic stress; however, T1 was not induced and T3 was only weakly induced. Western blot analysis showed induction of a predominant 55-kD T2 nuclear protein rather than the 68-kD T1 protein or the 48-kD T3 protein. Sequence analysis predicted, and EMSA and reporter analysis confirmed, that PA26 has a p53-binding site within intron 2. Serum starvation experiments suggested that PA26 may be a member of the growth arrest- and DNA damage-inducible, or GADD, gene family.
