Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SEMA4F in samples. An antibody specific for SEMA4F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The semaphorins comprise a large family of membrane-bound and secreted proteins, some of which have been shown to function in axon guidance. Encinas et al. (1999) cloned a novel semaphorin, which they referred to as semaphorin W (SEMAW). Sequence analysis of the SEMAW gene indicated that SEMAW is a member of the class IV subgroup of transmembrane semaphorins. The mouse and rat forms of semaphorin W share 97% amino acid sequence identity, and each shows approximately 91% identity with the human form. Expression studies showed that SEMAW mRNA is expressed at high levels in postnatal brain and lung and, unlike many other semaphorins, at low levels in the developing embryo. Functional studies showed that semaphorin W can collapse retinal ganglion cell axons.
