Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SDK1 in samples. An antibody specific for SDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.
