Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SCYE1 in samples. An antibody specific for SCYE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCYE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCYE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCYE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:AIMP1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.EMAPII is identical to the p43 subunit invariably associated with 9 aminoacyl-tRNA synthetase complexes. They stated that the C-terminal domain of p43 is an RNA-binding domain. EMAPII may be a bifunctional protein, binding RNA as part of the tRNA synthetase complexes and, after proteolytic cleavage in tumor cells, stimulating inflammatory responses.
