Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SCGB1D2 in samples. An antibody specific for SCGB1D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGB1D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGB1D2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB1D2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:LIPB is a member of the lipophilin subfamily, part of the uteroglobin superfamily, and is an ortholog of prostatein, the major secretory glycoprotein of the rat ventral prostate gland. Lipophilin gene products are widely expressed in normal tissues, especially in endocrine-responsive organs. Assuming that human lipophilins are the functional counterparts of prostatein, they may be transcriptionally regulated by steroid hormones, with the ability to bind androgens, other steroids and possibly bind and concentrate estramustine, a chemotherapeutic agent widely used for prostate cancer. Although the gene has been reported to be on chromosome 10, this sequence appears to be from a cluster of genes on chromosome 11 that includes mammaglobin 2.Highest expression was found in skeletal muscle. Expressed as well in thymus, trachea, kidney, steroid responsive tissues and salivary gland.
