Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate S1PR5 in samples. An antibody specific for S1PR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:According to these guidelines, a receptor is to be named with the abbreviation of the natural agonist with the highest potency, followed by a subscripted arabic number. The conceptualized 400-amino acid rat Edg8 protein shares 42 to 49% amino acid identity with the human S1P receptors EDG1, EDG3, and EDG5. Binding analysis confirmed that expression of Edg8 leads to high-affinity binding for labeled S1P.Widely expressed in the brain, most prominently in the corpus callosum, which is predominantly white matter. Detected in spleen, peripheral blood leukocytes, placenta, lung, aorta, and fetal spleen. Low-level signal detected in many tissue extracts. Isoform 1 is predominently expressed in peripheral tissues, whereas isoform 2 is expressed in brain, spleen and peripheral blood leukocytes.
