Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate S1PR4 in samples. An antibody specific for S1PR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:S1PR4 is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). Hence this receptor is also known as S1P4.This gene is a member of the endothelial differentiation, G-protein-coupled (EDG) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue.Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
