Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate S100A14 in samples. An antibody specific for S100A14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:S100A14 is a member of a subfamily of proteins related by Ca(2+)-binding motifs to the EF-hand Ca(2+)-binding protein superfamily.Using a proteomics approach to identify genes upregulated in breast cancer, followed by database analysis and PCR of a breast carcinoma cell line, Adam et al. (2003) cloned S100A14, which they called BCMP84. BCMP84 expression in normal tissue was restricted to stratified squamous epithelium of skin, cervix, and tonsil. Weak staining was seen in normal breast ductal tissue, but strong immunoreactivity was detected in 10 of 58 (17%) breast cancer tissues. BCMP84 was expressed in the plasma membrane of transiently transfected breast carcinoma cells.By fluorescence in situ hybridization, Pietas et al. (2002) mapped the S100A14 gene to chromosome 1q21 in a cluster with at least 15 other S100 genes.
