Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RILPL2 in samples. An antibody specific for RILPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRILPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RILPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RILPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:RILPL2 regulates cellular morphology. Knockdown of endogenous RILPL2 in neurons by short hairpin RNA (shRNA) interference results in reduced spine-like protrusions, a phenotype rescued by overexpression of an shRNA-insensitive RILPL2 mutant, suggesting a role for RILPL2 in both the establishment and maintenance of dendritic spines. RILPL2 and the Rho GTPase Rac1 form a complex, and that RILPL2 is able to induce activation of Rac1 and its target, p21-activated kinase (Pak). Notably, both RILPL2-mediated morphological changes and activation of Rac1-Pak signaling were blocked by expression of a truncated tail form of MyoVa or MyoVa shRNA, demonstrating that MyoVa is crucial for proper RILPL2 function. This might represent a novel mechanism linking RILPL2, the motor protein MyoVa and Rac1 with neuronal structure and function
