Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RGS6 in samples. An antibody specific for RGS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Members of the RGS (regulator of G protein signaling) family have been shown to modulate the functioning of G proteins by activating the intrinsic GTPase activity of the alpha (guanine nucleotide-binding) subunits.
RGS6, The predicted 472-amino acid protein has a calculated molecular mass of 54.4 kD. It contains an N-terminal dishevelled /egl10/pleckstrin (DEP) domain, a central G protein gamma subunit (GNG2)-like (GGL) domain, and an RGS domain near the C terminus. The variants encode proteins with long or short N-terminal domains, complete or incomplete GGL domains, 7 distinct C-terminal domains, and a common internal domain that includes the RGS domain.
