Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RGS5 in samples. An antibody specific for RGS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. The amino acid sequence deduced from the cDNA possessed all consensus motifs of the RGS domain and showed closest homology to mouse Rgs5, with which it was 90% identical, indicating that it represented human RGS5. The mRNA of the human gene is abundantly expressed in heart, lung, skeletal muscle, and small intestine, and at low levels in brain, placenta, liver, colon, and leukocytes.
