Detect Range:15.6-1000 pg/mL
Sensitivity:6.5 pg/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm

Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate REG3A in samples. An antibody specific for REG3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Regenerating islet-derived protein 3 alpha is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The predicted protein had the same size as the rat protein and showed 71% amino acid identity, the 6 half-cysteines being in identical positions. As in the rat, the protein was expressed at very low levels in control tissues and overexpressed during the acute phase of pancreatitis in humans. The protein could be immunolocalized to the apical regions of acinar cells. The protein was also quantifiable in serum with an elevation of levels in pancreatitis, suggesting its possible use as a biologic marker of the disease.