Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RBM8A in samples. An antibody specific for RBM8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM8A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:RBM8 encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events.
Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
