Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RBM24 in samples. An antibody specific for RBM24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:RBM24 is restricted to the lateral and ventral mesoderm during gastrulation and then localized to the somitic mesoderm, in a similar pattern as XMyoD gene. A 0.65kb X. tropicalis RBM24 regulatory region contains multiple E boxes (CANNTG), which are potential binding sites for MyoD and other bHLH proteins. Knockdown of Seb4 produces similar effects as those obtained by the dominant negative MyoD mutant, indicating that it is required for the expression of myogenic genes and myogenesis in the embryo. In cultured ectodermal explants, although overexpression of Seb4 has no obvious effect on myogenesis, knockdown of Seb4 inhibits the expression of myogenic genes and myogenesis induced by MyoD. Seb4/RBM24 is a direct target of MyoD and is required for myogenesis during Xenopus early development.
