Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate 17-OHP in samples. An antibody specific for 17-OHP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17-OHP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 17-OHP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17-OHP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:17-Hydroxyprogesterone is a C-21 steroid hormone produced during the synthesis of glucocorticoids and sex steroids. As a hormone, 17OHP also interacts with the progesterone receptor.It is derived from progesterone via 17-hydroxylase, a P450c17 enzyme, or from 17-hydroxypregnenolone via 3β-hydroxysteroid dehydrogenase/δ5-4 isomerase.
17-Hydroxyprogesterone is a natural progestin, and in pregnancy increases in the third trimester primarily due to fetal adrenal production. This hormone is primarily produced in the adrenal glands and to some degree in the gonads, specifically the corpus luteum of the ovary.17-Hydroxyprogesterone is not the same compound as 17-hydroxyprogesterone caproate. 17-Hydroxyprogesterone caproate is a synthetic (artificial) hormone that is similar in structure to medroxyprogesterone acetate and megestrol acetate.
