Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate 5-HIAA in samples. An antibody specific for 5-HIAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5-HIAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HIAA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HIAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin in the human body. In chemical analysis of urine samples, 5-HIAA is used to determine the body's levels of serotonin.
5-HIAA is tested by 24-hour urine samples combined with an acidic additive to maintain pH below 3. Certain foods and drugs are known to interfere with the measurement, and levels can vary depending on complications, such as a tumor, renal malfunction, small bowel resection, among other things. Since 5-HIAA is a metabolite of serotonin, testing is most frequently performed for the diagnosis of carcinoid tumors of the enterochromaffin (Kultschitzsky) cells of the small intestine, which release large amounts of serotonin. Values greater than 25 mg per 24 hours (higher if the patient has malabsorption) are strong evidence for carcinoid.
