Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate A1BG in samples. An antibody specific for A1BG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA1BG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A1BG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A1BG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Gahne et al. (1987) observed genetic polymorphism of A1B using one-dimensional horizontal polyacrylamide gel electrophoresis followed by Western blotting with specific antiserum. Three different phenotypes, designated 1-1, 1-2, and 2-2, were observed. Family data supported the hypothesis that the three phenotypes are determined by 2 codominant alleles at an autosomal locus.
In pigs the homologous locus is linked to malignant hyperthermia. Several other linkages in pigs and in horses suggest that human chromosomes 19, 6, and 1 are 'candidate chromosomes' for bearing the human A1B.
